[Histonet] Salivary gland tissue fetal pig problems
I want to get salivary gland tissue (parotid, submandibular, sublingual)
for the students. I thought I would try using the glands from the fetal
pigs (sent from Carolina Bio) we use for student dissection. I post-fixed
them briefly (~30 minutes) in 10%NBF, followed by
dehydration/infiltration. After sectioning, the connective tissue is
mostly degenerated and the gland tissue doesn't look that good either. The
good archival gland tissue we have is yellowish-colored, maybe it is fixed
in something different than NBF. Any suggestions? Thanks.
Melville B. Vaughan, Ph. D.
Department of Biology
University of Central Oklahoma
Edmond, OK 73034
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