[Histonet] Re: Water appropriate for IHC

From:Brian Chelack (by way of histonet)

A good rule of thumb for our lab is to use pyrogen free water for any application where enzyme activity is critical. We have a good ultrapure water system in our glassware and media prep lab, but every once in a while we had unexplainable declines in staining intensity. The only explanation that we could come up with was that these systems do not remove all pyrogens from the water(these can be small < 10,000 mw non-charged molecules that can inhibit HRPO or Alk phos reactions. These molecules can vary seasonally in the feedstock water, and their concentration in your lab water will also be dependent on how well the water purification system is maintained. Since we have changed over to using pyrogen free water (used for surgical irrigation), we have had fewer of these types of unexplained staining isses (not to say that we don't have other staining issues though...) This type of water is not cheap, but neither are stain repeats. Brian Chelack Prairie Diagnostic Services _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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