[Histonet] Re: Tissue with Fat
|From:||"Arshia Mian" (by way of histonet)|
I have been having some problems with cutting Tissue with Fat on a
cryostat (box temp and
object temp are at -20C). The slices I cut tend to curl in the middle
of the block close to the tissue. I have
changed the blade several times, but it still continues to happen even
with a new blade. Is there any correlation
between the box temp and the object temp that would cause this to
happen? Any suggestions?
I have also noticed that at times, even though my setting is set to 20
microns, that I get thick and thin cuts.
Any suggestions on this as well? I would greatly appreciate it. Thanks.
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