[Histonet] RE:immunofluorescence

From:"Pixley, Sarah (pixleysk)" (by way of histonet)

Dear Nita:
We currently are fairly happy with Fluoromount G, a water-miscible,
permanent-drying mounting medium purchased from Southern Biotech, Cat
#0100-01. But Fisher also sells a couple of similar media that do help
decrease the fading (Permount and Gel/Mount). They all vary a little,
but not by a huge difference. They just slow the photobleaching to a
degree. Then we store slides at room temperature in the dark. No
advantage to refrigerating and it certainly won't dry quickly in the
fridge. You remove excess mounting medium on top and sides of coverslip
with water.  I have looked at slides after a year or so and if the
staining was sufficiently bright to begin with, you can still see some.
But I wouldn't want to take pictures then.

I don't know one that is used to mount from xylene. But the last time I
did that, I just used a standard Depex mounting medium. I think the
fading was not too bad with that.

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