[Histonet] RE: Nissl Staining on Thick Sections

From:"Melissa Gonzalez" (by way of histonet)

Paul, have you tried Molecular Probes? Their Nissl stains work great, you
just have to adj. [ ]'s to your needs. Super fast and easy, and very
bright specific results.


Message: 2
Date: Tue, 18 Oct 2005 11:34:24 -0400
From: "Monfils, Paul" 
Subject: [Histonet] Nissl Staining on Thick Sections
To: "'histonet@lists.utsouthwestern.edu'"

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Does anyone routinely do Nissl staining on 30 to 40 micron frozen sections?
That's what the current project calls for, and I am finding that Nissl
methods designed for 6 to 8 micron sections don't work well on the thick
sections. They don't differentiate properly.  If anyone has a Nissl protocol
that works well on such thick sections, I would be grateful for any

Paul M.

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