[Histonet] Kappa, Lambda

From:"Jesus Ellin" (by way of histonet)

 I have a great question out there for the IHC people.  Here in the lab we
are trying to work up our Kappa and lambda.  The problem that most people
see is the excessive background staining that comes with these two
antibodies.  we are treating our tissue in a H2O2 solution just before we
run them on the IHC stainer (Ventanna).  Here is the kicker in working them
up we are using Bone marrow for control, because our pathologists would
like ,control that  is similar to the disease process they are looking for.
Now one specimen control stains beautiful for Kappa and the other specimen
control stains beautiful for Lambda.  This brings me to the conclusion that
the stain is working  and also that  different specimens no matter what
stain differently.  Can someone give me a universal way to make this easier
on us.  I know we need to block the endogenous peroxidase, and we are using
H2O2.. is there anything that we can do better or is there another method
for us to treat the specimens.

Jesus Ellin
Yuma Regional Medical Center

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