[Histonet] How far will stain reach into a thick section on a slide?

From:CM Bush (by way of histonet)

Dear Histonet,

I am wondering what you can tell me.  We are staining thick sections of
human brain.  The brain has been very well fixed in 10% NBF, and sections
are mounted on slides.  We are using thick sections (50um) and Nissl or
immuno staining.  Does any one know how deeply Nissl stain (or an IHC
stain) can permeate tissue mounted on a slide?  Is, say, any tissue
thickness greater than 20um (for example) moot as it will never be reached
by the stain?
Would staining floating sections be a more rational way to deal with thick

Thank you vey much for the help.

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