[Histonet] 37% formalin or penfix and false negative ER/PR stains
john eckman (by way of histonet)
Can anyone comment on what effect using 37% formalin to fix breast tissue
has on ER/PR immuno stains? Same question regarding alcoholic formalin use.
We work with pathology residents and have never ending problems with under
fixed breast tissue. Some have suggested using 37% formalin or an
alcoholic formalin. We're concerned about these fixatives altering immuno
stains, although some in our department have used 37% formalin elsewhere
without stain issues.
Our breast sections usually fix in 10% buffered formalin for 6 to 24 hours
before processing and we still get underprocessed/underfixed blocks in
histology. These blocks are then sent through the tissue processor again
(new Themo processor). Could the re-processing alter our ER/PR stains as
Appreciate any comments.
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