[Histonet] 37% formalin or penfix and false negative ER/PR stains

From:john eckman (by way of histonet)

Can anyone comment on what effect using 37% formalin to fix breast tissue has on ER/PR immuno stains? Same question regarding alcoholic formalin use. We work with pathology residents and have never ending problems with under fixed breast tissue. Some have suggested using 37% formalin or an alcoholic formalin. We're concerned about these fixatives altering immuno stains, although some in our department have used 37% formalin elsewhere without stain issues. Our breast sections usually fix in 10% buffered formalin for 6 to 24 hours before processing and we still get underprocessed/underfixed blocks in histology. These blocks are then sent through the tissue processor again (new Themo processor). Could the re-processing alter our ER/PR stains as well? Appreciate any comments. John --------------------------------- Yahoo! Music Unlimited - Access over 1 million songs. Try it free. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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