>>> "Cazares, Ruth"  10/19/2005 9:30 AM >>>
Janice,

I had a similar problem when we first got our Ventana instrument. What
it turned out to be the slides. We ordered from a cheaper source and got
spotty staining.  We switched to the Fisher (+) slides and have not had
a problem for the last 8 months.

Good Luck!

Ruth Cazares
Swedish Covenant Hospital
Chicago, IL


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice A
Mahoney
Sent: Tuesday, October 18, 2005 8:17 PM
To: histo20@hotmail.com; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana immunostainer

What kind of water are you using to make your solutions?
Jan
Omaha

>>> "Paula Wilder"  10/18/2005 6:12 PM >>>
Our institution is trying the new Ventana immunostainer.  We have had
the
unit since July and everything was staining nicely.  Then we started to
get
variable staining, especially with CK20, ER and PR, ki67.  Ventana reps
have
been working diligently to get this problem resolved.  We are using
pre-dilutes and the staining seems to be fine the first time, then
increasingly variable.  The reps took our controls to another area
hospital
and tested them there and the staining was fine; hence, the problem is
with
our machine.  I know they will replace this instrument if it continues
to
fail, but I was wondering if anyone out there has similar problems.
Thanks
for any help!

Paula Wilder
St. Joseph Medical Center
7601 Osler Drive
Towson, MD 21204
410-337-1741



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