-----Original Message-----
From: Sasa Jovanovic [mailto:sasa@jovanovic.co.za] 
Sent: 24 October 2005 04:00 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Re: Immunofluorescence problem - Non-specific


Dear Ms Callis,
Regarding the above mentioned subject I would like to ask if you could
possible assist me with some advice......In my project I am trying to
determine the presence of integrins in the rat endometrium by using
immunohistochemistry on frozen cryostat sections.
I am using DAKO ARK kit and was planning to add IgG2a secondary antibody as
Terry Johnson suggested in the above mentioned Histonet topic.....In what
stage of Dako Ark protocol and with what concentration to add this secondary
antibody?
Your advice will be mostly appreciated
Thank you in advance 
Sasha
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