FW: [Histonet] Kappa, Lambda

From:Patti Loykasek

Dear Jesus,
     Kappa and lambda are certainly difficult to interpret. They both 
can be
present circulating in the serum, thus the "background" staining in most
tissue sections. We use polyclonal antibodies, and get quite good 
We use a tonsil as a control as bone marrows are just too precious & 
for us to use. We look for the plasma cells to be staining with a dark
cytoplasmic stain & a slight "blush" to the germinal centers of the 
It does take a pathologist skilled in interpreting them. A true 
positive is
in the cytoplasm of the cells, not in-between the cells (due to 
K/L). We do use one titer for lymph nodes, and another for bone marrows 
& GI
biopsies.  Plus a different titer & pretreatment if we're looking for
Well, I hope I've helped & not confused you. I'd be happy to do my best 
answer any questions that you have.

Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA

> I have a great question out there for the IHC people.  Here in the lab 
> we are
> trying to work up our Kappa and lambda.  The problem that most people 
> see is
> the excessive background staining that comes with these two 
> antibodies.  we
> are treating our tissue in a H2O2 solution just before we run them on 
> the IHC
> stainer (Ventanna).  Here is the kicker in working them up we are 
> using Bone
> marrow for control, because our pathologists would like ,control that  
> is
> similar to the disease process they are looking for.  Now one specimen 
> control
> stains beautiful for Kappa and the other specimen control stains 
> beautiful for
> Lambda.  This brings me to the conclusion that the stain is working  
> and also
> that  different specimens no matter what stain differently.  Can 
> someone give
> me a universal way to make this easier on us.  I know we need to block 
> the
> endogenous peroxidase, and we are using H2O2.. is there anything that 
> we can
> do better or is there another method for us to treat the specimens.
> Jesus Ellin
> Yuma Regional Medical Center
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