Try increasing the cutting angle; cutting more slow and cooling the cutting surface inmediately before the section you are going to take.

cynthia haynes  wrote:Hello, everyone I am in need of information. I am
cutting brain(human)tissue at 6 microns and 20
microns. The 6 microns sections are perfect. The 20
microns sections are an nightmare(and I mean Elm
street nightmare). Can anyone suggest the best way to
cut these sections without getting wrinkles and folds.
I am at my wits end.
Thanks in advance

Cynthia Haynes H.T. 

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