[Histonet] Sticky Slides
Hi Histonet,
I am doing an in situ PCR/Hybridization. I am working with some cells
that were suspended in histogel, fixed in formalin, processed and
embedded. I am having some trouble keeping the tissue on the slides
through all of the incubations and wash steps. I am using silanized
slides and baking the slides at 60 degrees for 1 hour prior to use. Does
anyone have any suggestions as to how I can get these samples to stick
to the slides? Secondly, would a cytospin preparation be more likely to
stay on the slide? Thanks in advance.
-Vernon
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