[Histonet] Question about fixation

From:"Trajkovic, Dusko"




I received this email from a colleague. Can someone help him out????
Thank you,
Dusko Trajkovic
858-638-6202


Just wanted to send you a quick summary of my question from yesterday:

I wanted to know how to best fix late term mouse embryo heads, such 
as e14.5 and onward, esp injected with a virus that expresses 
GFP.  What I would ideally like to do is to cut, mount, stain with 
DAPI, and directly visualize by fluorescence 
microscopy.  Immersion-fixation in 4% PFA does not seem to work that 
well in general for head fixation.  Would formalin immersion-fixation 
work better?  Or other alternative fixatives?  What about 
autofluorescence from these other fixatives?

Thanks and see you later,
Yun




Yun C. Yung
Graduate student
Chun Lab, Dept of Molecular Biology
The Scripps Research Institute
yyung@scripps.edu
www.scripps.edu/mb/chun 



----------------------------------------------------------------------
LEGAL NOTICE
Unless expressly stated otherwise, this message is confidential and may be privileged.  It is intended for the addressee(s) only.  Access to this E-mail by anyone else is unauthorized.  If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful.  If you are not an addressee, please inform the sender immediately.

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mai

<< Previous Message | Next Message >>