[Histonet] Metachromasia of mast cells in epoxy resin sections
From: | gillian.2.brown@gsk.com |
Hi Histonetters,
would any one have experience of counting granules in mouse mast cells in
tissues which have been formaldehyde fixed, decalcified in a formic acid
based solution, post osmicated and embedded in epoxy resin? I'm taking 1
micron sections and looking to assess the 'degranulation' status of each
mast cell in terms of how many are still dark blue and how many are pink
ie based on the metachromatic properties of toluidine blue. I suppose my
question is will the formic acid have altered the dye binding properties
so that I'll get erroneous results? I'm going to experiment with tissue
that does not normally have this step but any insights now would be most
welcome.
Many thanks
Gill Brown
GlaxoSmithKline Medicines Research Centre,
STEVENAGE,
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