Re: [Histonet] question about air bubbles

From:"Sarah Jones"

Hi Frank,
  Is the tissue under vacuum during paraffin infiltration?  Sarah

Sarah Jones, HT(ASCP)
Histology Lab
Dept. of Veterinary Integrative Biosciences 
College of Veterinary Medicine
Texas A&M University
College Station, TX 77843-4458
phone: 979-845-3177
fax:  979-458-3499

>>>  10/29/2004 9:50:59 AM >>>
Hello everybody,
I am trying to serial section museum specimens of whole 
turtle embryo heads.  Specimens are infiltrated and embedded 
with paraplast X-tra.  The problem I am having is that there 
is air trapped in the specimen.  This makes the turtle head 
float in the molten paraplast.  The areas with air are not 
completely infiltrated with paraplast and section poorly; the 
delicate tissue collapses on itself.  If anybody can make any 
suggestions about how to remove the air and get complete 
infiltration, I would appreciate it.

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