Re: [Histonet] FETAL BRAIN FIXATION

From:"Bryan Hewlett"

Peggy,

We faced the same issue a number of years ago.
Our solution was to use a modified Brasil fixative.
The pediatric pathologist was delighted with the results!
The formula follows;

Modified Brasil Fluid.

Ethanol............................................. 700 mL
40% w/v Formaldehyde.....................100 mL
Glacial Acetic acid..............................50 mL
add D.water to make up to.................1000 mL

Fetal brains are left to fix for 1-3 weeks, rinsed in 70% ETOH, then sliced
and photographed.
Selected blocks are processed using the same reagents and times as for NBF
fixed material.

Hope this helps,

Bryan

----- Original Message -----
From: 
To: "Histonet" 
Sent: Thursday, October 21, 2004 4:56 AM
Subject: [Histonet] FETAL BRAIN FIXATION


> Anyone have a good fixative for the very soft fetal brains?
>
> Our neuropathologist does not want to use Bouin, as the tissue turns
yellow
> and photographs do not look the way they are supposed to.
>
> Another one of our pathologists remembers using an
alcohol-acetone-formalin
> mixture years ago as a resident in another institution, but doesn't know
the
> percentage of any of the reagents.
>
> I've tried several search engines and PubMed, and can't find anything.
>
> Thanks.
>
> Peggy A. Wenk, HTL(ASCP)SLS
> William Beaumont Hospital
> Royal Oak, MI 48073
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>