Re: [Histonet] Eosin, phloxine and OG?

From:John Kiernan

If you look at your H&E slides between staining and 
clearing & coverslipping you will find that eosin Y 
(CI 45380) can impart more than 3 colours to RBC,
smooth muscle cytoplasm, other cytoplasms and

Adding phloxin or orange G to an eosin Y counterstain
may extend the spectrum, but don't expect clear-cut
results from slides stained by a machine. 

The H&E method needs human judgement and adjustments
before applying a coverslip. For this reason and
others H&E is not a good "routine" staining method.
It's all there in the peer-reviewed literature and
in most histotechnology textbooks less than 25
years old. 
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
> (for H+E staining)?
> The conversation came up recently to "amp" the three shades of red in H+E
> staining.  It was the first time I'd ever heard of adding Orange G into
> eosin/phloxing solution. Are there any labs doing this?
> Thanks:-)
> Deb
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