Re: [Histonet] Antigen Retrieval w/ B&D Steamer.....

From:"Dina Floyd"

This is how we do it...
If you are using Citra, preheat Citra for 30 minutes, you can do this while 
your slides are running down to water. Put slides in for 20-30 minutes, Cool 
down to 40 degrees before rinsing in APK.
If you are using Trilogy, we start with two coplin jars of trilogy. Place 
your slides in the first jar as soon as they come out of the oven (DO NOT 
RUN DOWN TO WATER) Place coplin jars side by side in steamer and heat for 30 
minutes. Remove slides from first trilogy and transfer to second trilogy 
that has been preheating beside first trilogy. Steam another 30 minutes. 
Remove slides and immediately wash in several changes of apk wash.
-- Original Message ----- 
From: "Brandon Stokes" 
Sent: Friday, October 29, 2004 6:32 PM
Subject: [Histonet] Antigen Retrieval w/ B&D Steamer.....

> Hi fellow Histonetters........
> I have a quick question for anyone out there that knows anything about 
> using a Black&Decker Steamer for Antigen Retrieval.
> How long do you pre-heat the solutions?
> How long do you leave in the steamer?
> How long do you cool down?
> Those are a few of the questions that I have so far......If there is any 
> other helpful advice using this method it would be greatly appreciated.
> Thanks,
> Brandon Stokes, HT(ASCP)
> Lab Manager
> Cutting Edge Histology Services, LLC
> Tigard, OR
> 503-443-2157
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