Re: [Histonet] Antigen Retrieval w/ B&D Steamer.....

From:Kim Merriam

Hello,
 
This is what I do:
 
1. Preheat retrieval solution in microwave for 6 minutes (3 tissue tek containers will fit into 1 steamer)
2. Preheat steamer for 10-15 minutes (you can do this while bringing slides down to water).
3. Heat slides for 45-60 minutes
4. Cool down for at least 10 minutes
 
Kim Merriam
Novartis
Cambridge, MA

Brandon Stokes  wrote:
Hi fellow Histonetters........

I have a quick question for anyone out there that knows anything about using a Black&Decker Steamer for Antigen Retrieval. 

How long do you pre-heat the solutions?
How long do you leave in the steamer?
How long do you cool down?

Those are a few of the questions that I have so far......If there is any other helpful advice using this method it would be greatly appreciated.

Thanks,

Brandon Stokes, HT(ASCP)
Lab Manager
Cutting Edge Histology Services, LLC
Tigard, OR
503-443-2157


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Kim Merriam
Novartis
Cambridge, MA
				
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