RE: [Histonet] re: bone decal - clarification

From:"Patsy Ruegg"

Decal can leach out the iron also.

-----Original Message-----
[] On Behalf Of Sharon
Sent: Monday, October 18, 2004 10:40 AM
Subject: [Histonet] re: bone decal - clarification

Hi Histonetters,

Thanks to everyone who has answered my bone decal question so far - 
I'm writing to add a clarification - the bone is mouse femur (pretty 
small and thin) about one year old.  I'm treating it in HCl because 
I'm putting it in Perl's solution with 4% paraformaldehyde first 
before anything else to preserve the iron, which I find leaches out 
during fixation.  It works well, probably because formalin is more 
stable in acid solution.  I'm just wondering if maybe I don' have to 
go through the decal afterwards.  I guess if the one hour in 1% HCL 
isn't enough for decal then I should use a regular decal step rather 
than continue in 1% HCl because that would better preserve antigens 
for subsequent IHC.

Sharon Cooperman        	     
NIH, NICHD, CBMB                     301.435-8417
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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