RE: [Histonet] PLP Fixation

From:"Mitchell (Jean)"

I use periodate-lysine-paraformaldehyde fixative for skin samples. I
start with a lysine stock solution & a paraformaldehyde stock solution
(each stable for 3 weeks). I make up the working solution with those
stock solutions the day of use adding the periodate powder at that time.

I have not experienced the solution turning yellow after 24 hours. If
you would like more specifics on the solutions I use, I would gladly
forward that information to you.

Jean Mitchell, BS, HT (ASCP)
University of Wisconsin Hospital & Clinics
Department of Neurology
Madison, WI

-----Original Message-----
[] On Behalf Of Cheryl
Sent: Wednesday, October 20, 2004 4:52 PM
To: Histonet
Subject: [Histonet] PLP Fixation

We have a research project in which all the tissues are fixed in
periodate-lysine-paraformaldehyde (PLP).  The question has arisen as to
how long in advance to use can the solution be made? and the solution
yellow after approx. 24 hours.  Is this solution still usable?   Thank
for your help.  

Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Anatomic Pathology
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Skip Bertman Drive
Baton Rouge, LA  70803
FAX:  225-578-9720

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