Wayne,

I am sure the Histonet archives will give you a large amount of information
about this.  We had problems as well and have been in correspondence with
the Menzel Group in Germany about this.  They are looking into the problem
and agree there may be a problem with some batches (this appears to be
worldwide).  Is it only with slides which have had EDTA retrieval?   We had
slides on a parallel run, with the same pretreatment and antibody, which
have stained with haematoxylin only, others with no staining with anything,
others with partial staining across the section.

The people from Menzel asked us to send them slides, both stained! and
unused, from the batch (always only one batch) which gave these results,
they agree that there is something peculiar about them.

It happens infrequently this is the second time in two years and not all
slides are affected.

Ah the joy of being a Histotech

Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Wayne
Holland
Sent: Thursday, 21 October 2004 2:00 AM
To: wayneholland1959@msn.com; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Hydrophobic properties on red control box
slides.


Let me make it clear that the manufacter is working closely with me to
resolve this problem, they have offered much help in this matter. They are
also examining the lots that were used. Maybe I thought I was crazy. Your
input please!

>From: "Wayne Holland" 
>To: Histonet@lists.utsouthwestern.edu
>Subject: [Histonet] Hydrophobic properties on red control box slides.
>Date: Wed, 20 Oct 2004 11:44:16 -0400
>
>Has anyone experienced using an automated immuno system that maintains the
>slides on a horizontal plain during the run, having possible hydrophobic
>properties. This prevents the antibody and other reagents from being
>dispersed evenly on the slide. The reagents on this system are dispensed in
>the area of the control box (with the control box being painted on the
>underside of the slide). However when these slide are shipped, they are
>boxed back to front laying on one another of course, this causing the paint
>properties to be transferred to the slide in front if it. I have found that
>I have had to run the coated slides through Xylene and alcohol, then air
>dry under a hood before we mount the tissue. Has anyone experienced this
>being a problem? Once we started this additional step, all the problems of
>the tissue in the control box staining and not the patient has been
>eliminated. PLEASE HELP. The people that make the glass think I'm crazy.
>
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