RE: [Histonet] Eosin, phloxine and OG?
From: | "Marshall Terry Dr, Consultant Histopathologist" |
Come on John - you can't get away with half a story:-)
If H&E isn't a good routine stain, what is (and how many years to get used to it)?
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Consultant Pathologist
Rotherham General Hospital
South Yorkshire
England
terry.marshall@rothgen.nhs.uk
-----Original Message-----
From: John Kiernan [mailto:jkiernan@uwo.ca]
Sent: 20 October 2004 06:26
To: WWmn916@aol.com
Cc: histonet@pathology.swmed.edu
Subject: Re: [Histonet] Eosin, phloxine and OG?
If you look at your H&E slides between staining and
clearing & coverslipping you will find that eosin Y
(CI 45380) can impart more than 3 colours to RBC,
smooth muscle cytoplasm, other cytoplasms and
collagen.
Adding phloxin or orange G to an eosin Y counterstain
may extend the spectrum, but don't expect clear-cut
results from slides stained by a machine.
The H&E method needs human judgement and adjustments
before applying a coverslip. For this reason and
others H&E is not a good "routine" staining method.
It's all there in the peer-reviewed literature and
in most histotechnology textbooks less than 25
years old.
--
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan[AT]uwo.ca
http://publish.uwo.ca/~jkiernan/
http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
> (for H+E staining)?
> The conversation came up recently to "amp" the three shades of red in H+E
> staining. It was the first time I'd ever heard of adding Orange G into
> eosin/phloxing solution. Are there any labs doing this?
>
> Thanks:-)
> Deb
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>