RE: [Histonet] Dog antibody search

From:"Goodpaster, Tracy A"

Hello Jackie O'

I use Cleaved Caspase-3 from Cell Signaling (Clone Asp175, cat.#:9661L) on
dog lymph node and gut with great success.  I use it at 1:100 with Dako
Target Retrieval Solution pH 6.0.  I use the same solution for the serum
block as antibody diluent, which is a mixture of Dako washing buffer and 20%
serum (10% goat serum, 5% human serum, 5% dog serum).  The secondary is
Vector's Biotinylated goat anti-rabbit BA-1000.  The tertiary is Vector's
R.T.U. Vectastain Kit (PK-7100).  I tried Dako's Envision kit (great for so
many antibodies) but it gave a slightly weaker stain that was not as crisp.
The DAB+ and Hematoxalin were from Dako (K4011 and S3301 respectively).
I hope this will help you.

Tracy Goodpaster     

-----Original Message-----
From: Jackie.O' [mailto:Jackie.O']
Sent: Tuesday, October 19, 2004 5:01 AM
To: Histonet
Subject: [Histonet] Dog antibody search

Hi guys - 
Can anyone steer me towards a supplier for Caspase-3, MPM-2, and Ki-67 
primary antibodies that will work for IHC on FFPE Dog??

Thanks much!

Jackie O'
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