Ann,

CD15 antibodies a very finicky.
Being an IgM antibody, you might need to use a anti-mouse IgM after the
localisation antibody to improve the sensitivity. I have also found a
difference in clones with Leu-M1 from Becton-Dickinson better than Dako's
C3D-1.

Often overnight incubation with the Dako antibody will also improve the
sensitivity.


Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318


-----Original Message-----
From: ANN MARUSKA [mailto:amarusk1@FAIRVIEW.ORG] 
Sent: Tuesday, 26 October 2004 11:14 PM
To: histonet@pathology.swmed.EDU
Subject: [Histonet] CD15


Hi Histonetters,
 
We have recently had problems where our CD15 is NOT staining in any of
the Hodgkins cases we had - although, the CD30 is staining quite well. 
Our control will stain well but we have had 3 patients who have stained
negative with our procedure and yet positive at another institution.
This has been on both bone marrow cores and nodes.
 
We currently use Ventana's pre-dilute (clone MMA) on our Benchmark.
 
Has this happened to anyone else - or do you have any suggestions?
 
Thanks in advance.
 
Ann
 
Ann Maruska
Fairview-University Medical Center
Mpls. MN  55454
amarusk1@fairview.org
612-273-9119

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