[Histonet] re: microwave retrieval
I previously used plastic coplin jars set into a tupperware-type square
dish, but also had occasional evaporating/boiling down of buffer and
subsequent uncovering/drying out of tissues (even though I'd check and add
to the buffer level after every 5 minutes).
Now I use Tissue-Tek plastic containers, the rectangular-shaped ones that
are used in the deparaffinization/rehydration Tissue-Tek system. They hold
more buffer volume, and it seems that less buffer is evaporated/boiled off.
I still check buffer levels after every 5 minutes of microwaving, but have
never lost too much buffer volume since switching. And, the added bonus is
that each container can hold up to 24 slides in a Tissue Tek plastic slide
Univ. of Minnesota
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