[Histonet] human Ab on human tumor
Currently I am doing IHC using human Ab on human tumor. I know it would cause "messy" background. But this tumor was developed by injection human tumor cell to nude mice. The results came out pretty good. I didn't use conjugated primary Ab, but just traditional method. I think it's because the tumor was developed in mice, the tissue would not contain endogenous human serum that causes the "messy background", although the tumor is human tumor. Am I right on this? Or my stain result just "fake"?
Hope I said it clearly. Could any expert give me help?
Thanks in advance!
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