[Histonet] Sectionnong whole-body rats with a BRIGHT 8250 Cryostat
I'm using a Bright 8250 cryostat to do rat whole-body sections for
QWBA analysis. Since I started using the microtome, (even with blood
standards blocks or a rat carcass) sometimes (1 time/10), the knife
appears to slip on the block at the begginning of the block and start
to section at the end. I tried two type of knife (D-profile and
tungsten) and many angles (generaly 10 degree for the D-profile and 20
I tried to adjust the slide tensioners. I tried two types of CMC. I
tried different temperature (-20 c, -25 c) and it still doesn't work.
WHAT IS THE PROBLEM????
Please, HELP ME, I'm trying to solve this problem since many months.
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