[Histonet] RE: Histonet Digest, Vol 11, Issue 30
Hello. Does anyone have any experience working with CD41, GPV, and GPIBbeta
antibodies?
I am looking for protocols to use them for immunohistochemistry on mouse
embryos fixed in paraformaldehyde. THANK YOU!
Rachael L. Emerson
Center for Human Genetics and Molecular Pediatric Diseases
University of Rochester Medical Center
575 Elmwood Avenue MRBX 1.11301
Rochester, NY 14642
Tel (585) 275-5073
Fax (585) 276-0232
> ----------
> From: histonet-request@lists.utsouthwestern.edu
> Reply To: histonet@lists.utsouthwestern.edu
> Sent: Friday, October 22, 2004 1:03 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 11, Issue 30
>
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> Today's Topics:
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> 1. (Fwd) Re: [Histonet] Giardia Primary Antibody (Greg Dobbin)
> 2. Re: Microwave retrieval (mark.lewis@thermo.com)
> 3. CAP question (Goeden, Catherine)
> 4. RE: Microwave retrieval (amosbrooks@earthlink.net)
> 5. reference for plastics (Patrice McCall)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 22 Oct 2004 08:47:07 ADT
> From: "Greg Dobbin"
> Subject: (Fwd) Re: [Histonet] Giardia Primary Antibody
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <4178C90A.30772.E384F@localhost>
> Content-Type: text/plain; charset=US-ASCII
>
>
> ------- Forwarded message follows -------
> From: Greg Dobbin
> To: "Etheridge, Sandra AGF:EX"
>
> Subject: Re: [Histonet] Giardia Primary Antibody
> Date sent: Fri, 22 Oct 2004 08:46:21 ADT
>
> Hi Sandra,
> Here at AVC we just had a young parasitologist leave for a job in
> Perth, Australia, his name is Dr. Ryan O'Hanley and giardia is his
> thing. He was using an Ab for IFATs.
>
> He bought his Ab's from Waterbourne Inc. in New Orleans. He
> ordered "Giardia/A/Glow" Cat # A300. Sounds like a FITC
> conjugated Ab, but it's a start. Have a nice weekend.
> Greg
>
> From: "Etheridge, Sandra AGF:EX"
>
> To: " (histonet@lists.utsouthwestern.edu)"
>
> Date sent: Thu, 21 Oct 2004 14:04:47 -0700
> Subject: [Histonet] Giardia Primary Antibody
>
> > Hello everyone,
> >
> > We are looking for a primary antibody for Giardia. Is anyone out there
> in
> > the veterinary research community aware of anyone who will supply us
> with
> > some?? I have checked out numerous websites and found one lab who tests
> for
> > it in Michigan (from the South Dakota State IHC web page), but have not
> > heard back from them.
> >
> > Any info is, as always, appreciated.
> >
> > Thanks!
> >
> > Sandra Etheridge
> >
> > BC Ministry of Agriculture, Food & Fisheries
> > Animal Health Center, Histology
> > Abbotsford, BC
> > Canada
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------- End of forwarded message -------
>
>
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Greg Dobbin
> Pathology Lab
> Atlantic Veterinary College, U.P.E.I.
> 550 University Ave.
> Charlottetown, P.E.I.
> Canada, C1A 4P3
> Phone: (902)566-0744
> Fax: (902)566-0851
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Happiness is a journey, not a destination.
>
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 22 Oct 2004 08:46:48 -0400
> From: mark.lewis@thermo.com
> Subject: Re: [Histonet] Microwave retrieval
> To: Patti Loykasek
> Cc: histonet-bounces@lists.utsouthwestern.edu, histonet
>
> Message-ID:
>
>
>
> Content-Type: text/plain; charset=iso-8859-1
>
>
> Patty,
>
> I think you hit the nail on the head when you said "I believe consistency
> in what you do is the key to any antigen
> retrieval." I believe it is important for all aspects of the Histo lab. It
> makes trouble-shooting problems much easier if the work is all done in a
> consistent manner.
>
> Have a nice day
>
>
> Best regards,
>
> Mark
>
> Mark Lewis
> Product Specialist
> Anatomical Pathology
> Clinical Diagnostics
> Thermo Electron Corporation
> (412) 747-4013
> (412) 788-1097
> E-mail: mark.lewis@thermo.com
>
>
>
>
>
> Patti Loykasek
>
> To:
> histonet
>
> Sent by: cc:
>
> histonet-bounces@lists.utsouth Subject: Re:
> [Histonet] Microwave retrieval
> western.edu
>
>
>
>
>
> 10/21/2004 12:09 PM
>
>
>
>
>
>
>
>
>
> Ed,
> I've done antigen retrieval many ways, and there are pros & cons to
> each. I believe consistency in what you do is the key to any antigen
> retrieval. I do not recommend microwaving with open containers - it is far
> too easy for there to be hot spots, loss of fluid, etc.... That being
> said,
> I do think it is possible to microwave retrieve in a closed container such
> as a microwave pressure cooker. Some of the keys to this technique are:
> use
> the same volume of liquid, the same # of slides (using blank slides if
> necessary) each & every time, monitoring with your microwave the time it
> takes to get up to pressure & how long you want your slides to remain
> under
> pressure, careful cooling down of slides. I1d be happy to provide the
> particulars of how we do it, if anyone is interested. In a microwave
> pressure cooker the fluid becomes 3super heated2 - much hotter than in a
> steamer & you have the added pressure. We have found it improves staining
> with many difficult targets & nuclear antigens.
> I would note that I work at a reference lab, and our tissue is fixed &
> processed many different ways.
> If you can control the fixation & processing, and optimize it, I think a
> steamer works great & we use it for many antibodies. Again, consistency is
> the key. We preheat solutions before adding slides, only adding slides
> when
> the solution has reached 95 degrees C. To monitor the temperature we have
> thermometers with slender, metal probes that fit thru the holes in the
> steamer ( alternately, I1ve had my husband carefully enlarge the steamer
> lid
> holes to accommodate a standard thermometer). After adding slides, we
> start
> our retrieval time when the solution is back up to 95. We do the same
> retrieval time & cool down time each time.
> Well, I hope this helps you out. Feel free to contact me for more info.
> Good
> luck with your retrieval & staining.
>
>
> Patti Loykasek
> PhenoPath Laboratories
> Seattle, WA
>
>
>
>
> > Can anyone give me the pro's and con's of steamer/pressure (Decloaker)
> vs.
> > microwave retrieval ?
> > Our Doc wants us to change from the Decloaker to microwave. Is a
> household
> > microwave adequate or do we need a lab grade unit?
> > Does the microwave save time and improve turn around time?
> >
> > Thanks in advance
> > Ed Harris HT ASCP MT ABB
> >
> >
> >
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> ------------------------------
>
> Message: 3
> Date: Fri, 22 Oct 2004 08:42:01 -0500
> From: "Goeden, Catherine"
> Subject: [Histonet] CAP question
> To: "'histonet@pathology.swmed.edu'"
> Message-ID: <597FCC5997E8504EAA6814E6C9ED52298FAD6A@VHASUXEXC1>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> We are getting ready for a CAP inspection and I have a question about how
> other techs are handling a question regarding waterbaths:
>
> ANP.23350 Are they clean and well-maintained, and is there a policy for
> preventing cross-contamination of paraffin sections in the bath?
>
> I have a procedure for cutting the sections and cleaning water baths but I
> guess I am not specifically using the word cross-contamination. Would
> appreciate how other labs are "wording" this procedure.
>
> Thanks in advance!!
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 22 Oct 2004 10:48:40 -0400 (GMT-04:00)
> From: amosbrooks@earthlink.net
> Subject: RE: [Histonet] Microwave retrieval
> To: "SMITH,REBEKAH FELICIA" ,
> histonet@lists.utsouthwestern.edu
> Message-ID:
> <10909363.1098456520591.JavaMail.root@skeeter.psp.pas.earthlink.net>
> Content-Type: text/plain; charset=us-ascii
>
> Hi,
> Just to point out a steamer is cheaper than a microwave. So for a lab
> on a budget the steamer is even better. You should give it a whirl some
> time. You may find some improved consistancy. Of course if you are content
> with what you have I stand by something I always say: All labs are
> different, What works fine in one lab may cause problems in another. If it
> aint broke don't fix it.
> Amos
>
>
> -----Original Message-----
> From: "SMITH,REBEKAH FELICIA"
> Sent: Oct 21, 2004 10:29 PM
> To: Luis Chiriboga ,
> "Weems, Joyce" ,
> marjorie lehman ,
> Amos Brooks ,
> histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Microwave retrieval
>
> Being myself a poor research tech, I also use the household
> microwave method. I stick my slides in coplin jars filled with
> Tris buffer with the tops off then stick them in another container
> and microwave for 45sec twice, changing the water in between. It's
> been working for me.
> SMITH,REBEKAH FELICIA
> "You are a child of the universe, no less than the trees and the
> stars
> You have a right to be here and whether or not it is clear to you,
> no doubt the universe is unfolding as it should. Therefore be at
> peace with G-d, whatever you conceive Him to be. And whatever your
> labors and aspirations,in the noisy confusion of life, keep peace
> in your soul.-Max Ehrmann,"Desiderata"
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 22 Oct 2004 09:28:14 -0700 (PDT)
> From: Patrice McCall
> Subject: [Histonet] reference for plastics
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <20041022162814.84913.qmail@web54504.mail.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> Does anyone know a good reference book or other
> material to use for learning to do plastics in histology?
>
>
>
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> End of Histonet Digest, Vol 11, Issue 30
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