[Histonet] RE: Eosinophil marker

From:"C.M. van der Loos"

Dear Brianna,
According to the datasheet of Monosan (www.monosan.com) antibody MON6008 (clone BMK-13) this antibody is staining FFPE sections after treating the tissue section with pepsin; not with HIER! Perhaps this pepsin treatment is worthwhile to test with your Chemicon antibody.
Hope this helps.

Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center
Amsterdam - The Netherlands

----- Original Message ----- 
>From  "UniPath IHC"  
Date  Wed, 27 Oct 2004 10:34:50 -0600 
Subject  RE: [Histonet] Eosinophil marker 
I guess I should have made myself clear, and I admit I forgot to
sign my name before I sent off the email.  I'm an IHC tech working for a
private company.  However, I'm not just trying to get "free advice on the
internet".  I have been a member of the Histonet community for four years
and have enjoyed reading and contributing to this service.  I would never
use a response from Histonet as anything but advice or a hint in the right
direction.  As for who I work for, I have never been told that working for a
private lab as opposed to a hospital excluded me from Histonet. 
I am working on a research project for a couple of doctors at the
National Jewish in Denver.  I am trying to doublestain eosinophils in skin
biopsies by IHC and am having no luck with the MBP antibody (Chemicon) on
routinely processed FFPE.  It works fine for me on frozen sections, and even
a little bit on tissue fixed in 4% PFA.
I found a few emails in the archives regarding eosinophil markers,
but most people asking what I need to know had never been replied to, at
least not via the Histonet.  I have considered special stains to mark
eosinophils, but the doctors I am working with would like to use IHC.
If I cannot make this antibody (MBP) work on FFPE then I need to
find another eosinophil marker that does.  Any help would be appreciated.

Thanks for your time,

Brianna Jackson, BS, QIHC
UniPath, LLC
Denver, CO

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