Hi,

has anyone experience with antibodies which detect the FLAG protein tag? I
want to analyze the expression of a FLAG-tagged transgene in brainstem
sections of mice. I have tried one FLAG-ab from Kodak/Sigma, but it didnīt
work (after perfusion with 4% PFA). I have not tried antigen-retrieval, due
to the fact, that I was quite unhappy with the tissue quality after the
procedure in the past.
There are other FLAG-abs (e.g. Chemicon MAB3118), but I am not interested in
testing them all.

Thanks for your help.

peter




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