[Histonet] Evans blue dye and myocardial infarction
I hope that someone out there may be able to shed light on our problem.
We are studying means to reduce the tissue damage caused by heart
attacks. Our studies suggest that, following the induction of a heart
attack in our sheep model, some of the surviving tissue is compromised.
We are attempting to delineate the areas of compromised tissue by
post-mortem infusion of 0.15% Evans blue dye in 0.9% saline (we have
also tried phosphate-buffered saline). The dye is infused into the
coronary arteries immediately after death, followed by flushing with
0.9% saline. Our understanding of Evans blue is that it cannot cross
intact cell membranes and therefore will only enter compromised muscle
fibres (it has been used in this way to mark dystrophic muscle fibres in
the mdx mouse). Can anybody tell me why the whole heart is staining blue
during infusion, and remaining so following flushing. This happens even
in control hearts which have not suffered a heart attack, and therefore
have only normal muscle.
I would be very grateful if anybody could come up with some suggestions.
Ken Matthews PhD
Functional Muscle Genomics Group
Private Bag 3123
Attention: The information contained in this message and/or attachments
from AgResearch Limited is intended only for the persons or entities
to which it is addressed and may contain confidential and/or privileged
material. Any review, retransmission, dissemination or other use of, or
taking of any action in reliance upon, this information by persons or
entities other than the intended recipients is prohibited by AgResearch
Limited. If you have received this message in error, please notify the
Histonet mailing list
<< Previous Message | Next Message >>