[Histonet] Any fixative to reduce background in immunofluorescence?
I am preparing to do some immunofluorescence staining in kidney tissue
Some preliminary work in formalin fixed paraffin embedded tissue has
yielded quite significant background fluorescence.
A colleague suggested that I use frozen tissue, but the morphology is
Another colleague suggested paraformaldehyde, but that is really
inconvenient (preparing this every morning!?)
Any advice on a good commercial fixative for this purpose, that doesn't
destroy vessel antigens?
This email message, including any attachments, is for the sole use of the=20intended recipient(s) and may contain confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient(s) please contact the sender by reply email and destroy all copies of the original message. Thank you.
Histonet mailing list
<< Previous Message | Next Message >>