SV: [Histonet] Mounting media to covberslip from ethano

From:=?iso-8859-1?Q?Eva_Alstr=F6mer?=

Dear Paul,

In Europé it is two different mounting mediums(containing xylene),
Mountex and Pertex in the market. Both give you possibility to mount
direct after an extra jar with clean alcohol, these two mountingmedium
are very fast drying and do not change in colour after storing of many
years.
Eva 

-----Ursprungligt meddelande-----
Från: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] För Paul Bradbury
Skickat: den 5 oktober 2004 18:17
Till: HistoNet Server
Ämne: Re: [Histonet] Mounting media to covberslip from ethano


I have never encountered a mounting medium that uses alcohol as the 
solvent.

If one exists, two disadvantages spring to mind immediately. One, the 
refractive index of the medium would be low, so the clarity and 
brilliance associated with conventional, xylene-based media would be 
absent. Two, a large proportion of counterstains (eosin, van Gieson, 
neutral red, methylene blue, etc) are alcohol -soluble and would "bleed"

into the medium.

Paul


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