Dear List-fellows



I want to thank all of you that answered to my query about quick decalcification.



best wishes



José Luis









El dia 12/10/2004 19:13 usted envio el siguiente mensaje:

>Date: 12 de Octubre de 2004 19:13:01

>From: "Patsy Ruegg"  

>Subject: RE: [Histonet] bone decalcification 

>To: jluis.palazon@icman.csic.es, histonet@lists.utsouthwestern.edu

>

> Jose,

> If you are thinking of doing IHC Formic Acid is a good decal to use

> (5%).  Decal Chemicals sells a formic acid product called Immunocal.

> There are other quicker products that I believe use Nitric acids but IHC=0D
> is better with the Formic in my hands.

> Patsy

> 

> -----Original Message-----

> From: histonet-bounces@lists.utsouthwestern.edu

> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jose

> Luis Palazon Fernandez

> Sent: Tuesday, October 12, 2004 3:34 AM

> To: histonet@lists.utsouthwestern.edu

> Subject: [Histonet] bone decalcification

> 

> 

> Dear List-members

> 

> 

> 

> I have to dacalcify some fish vertebrae in order to see the vertebral

> articulation and intervertebral discs, and have to use a quieck method.=0D
> I usually decalcify with EDTA but this method is very slow. I would

> appreciate your advise. 

> 

> 

> 

> Thanks in advance!

> 

> 

> 

> José Luis

> 

> Universidad de Oriente-Isla Margarita-Venezuela

> 

> actualmente en: Instituto de Ciencias Marinas de Andalucia

> 

> Puerto Real, Cádiz, España.

> 

> email: jluis.palazon@icman.csic.es

> 

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> 







Universidad de Oriente-Isla Margarita-Venezuela

actualmente en: Instituto de Ciencias Marinas de Andalucia

Puerto Real, Cádiz, España.

email: jluis.palazon@icman.csic.es

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