To Atoska,
My experience shows that PFA isn't good enough as a fixative. I fix the tissue  again in 10% NBF for 6-12 hours, and process as usual. Try it. It really is much better.
Jo

>>> "Atoska S. Gentry"  10/06/04 03:08PM >>>

Hello, those of you processing & sectioning coronal sections of mouse 
brain; from you experience can prolonged fixation in 4%PFA  be the culprit 
of sectioning problems? What is the maximum fixation time period the brain 
should held in PFA before processing & sectioning? Thanks! Atoska


Atoska S. Gentry B.S., HT(ASCP)
Research Assistant III
Scott-Ritchey Research Center
College of Veterinary Medicine
Auburn University, AL  36849
Phone# (334)844-5579  Fax# (334)844-5850 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet