Re: [Histonet] fluorescent staining of nuclei or nuclear material

From:"J. A. Kiernan"

Fluorescence microscopy is useful for seeing
small numbers of bright objects against a
dark background. An ordinary Feulgen stain,
though done for ordinary microscopy,
is also fluorescent. It is specific for DNA
if you do the proper controls.

Any fluorescent yellow cationic dye will
stain nuclei. Acriflavine (about 0.001%)
is cheap and effective. Bisbenzimide
(also called Hoechst 33258) is more often
used; it can be incorporated into an aqueous
mounting medium.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
Elizabeth Chlipala wrote:
> Hello everyone
> I have a client that has some tissue (fascia) samples which have been
> processed in an attempt to remove the intrinsic cells.  They have some
> H&E sections, but want to use a more sensitive technique to ensure that
> no cell remnants or nuclear material remains.  Is there is a fluorescent
> staining method to visualize cell nuclear material.  I have gone to the
> molecular probes web page and there is a wide variety of nuclear stains
> available.  Does anyone have some advice on what would be the best
> method for this application and whether or not it would work on paraffin
> embedded material.
> Thanks in advance.
> Liz
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Histology Laboratory, LLC
> P.O. Box 18592
> Boulder, Colorado 80308
> Office: (303) 735-5001
> Fax: (303) 735-3540
> Ship to Address:
> Premier Histology Laboratory
> University of Colorado
> MCDB, Room A3B40
> Boulder, Colorado 80309
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