Re: [Histonet] Smears

From:Teresa Dominguez

What I do with old smears is :
  To place the smears in a Fisiologic solution for , at least 10 minutes,
and then re-fixation in  95% alcohol, after that you can stian the smean
with the technicque you want.
Good luck!

Ht. Maria Teresa Dominguez
Patology Service
Rio Grande Reg. Hospital
Rio Grande, Tierra del Fuego,
----- Original Message -----
From: "Baowei Peng" 
Sent: Friday, October 08, 2004 4:44 AM
Subject: [Histonet] Smears

> Dear all histoneters,
> Are there anyone can help me out of the smears staining problem.
> I\'m doing lymphocyte smears staining these days as following:
> smears were made from lymphocyte which were in 1640. After air dry, smears
were dip into methanol for several times and were stained in Giemsa satin to
indetify macrophages. The result shows that almost holf of the cells in the
smears have broken and leak out their cytosol and nucleus contents.
> What I have done wrong and what can I prevent the cells from broken?
> Yours sincerely,
> Baowei Peng
> Pharmacy School
> Shanghai Jiaotong University
> Shanghai, 200030
> China


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