RE: [Histonet] bone marrow for immunostaining - how?

From:Kirbis Srebotnik Irena

you can either suspend bone marrow in tissue culture medium, prepare
cytospins (fix in methanol) and stained them by immunocytochemistry or you
can perform flow cytometry immunophenotyping on this cell suspension.

Irena Srebotnik Kirbis, MSc
Institute of Oncology
Dept. of Cytopathology
Zaloska 2
1000 Ljubljana
Slovenia
phone +386 1 522 3826
fax +38615879400


-----Original Message-----
From: Julia Edgar [mailto:je22r@udcf.gla.ac.uk]
Sent: Monday, October 11, 2004 10:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] bone marrow for immunostaining - how?


Dear All
I need to collect some bone marrow to test out an antibody to CD45. Can you
please tell me how to go about it and how to store the material once
collected.
Thank you in advance
Julia
Julia Edgar, BSc (Hons), PhD
University of Glasgow Veterinary School
Glasgow G61 1QH
e-mail: je22r@udcf.gla.ac.uk
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