RE: [Histonet] bone decalcification

From:"Patsy Ruegg"

If you are thinking of doing IHC Formic Acid is a good decal to use
(5%).  Decal Chemicals sells a formic acid product called Immunocal.
There are other quicker products that I believe use Nitric acids but IHC
is better with the Formic in my hands.

-----Original Message-----
[] On Behalf Of Jose
Luis Palazon Fernandez
Sent: Tuesday, October 12, 2004 3:34 AM
Subject: [Histonet] bone decalcification

Dear List-members

I have to dacalcify some fish vertebrae in order to see the vertebral
articulation and intervertebral discs, and have to use a quieck method.
I usually decalcify with EDTA but this method is very slow. I would
appreciate your advise. 

Thanks in advance!

José Luis

Universidad de Oriente-Isla Margarita-Venezuela

actualmente en: Instituto de Ciencias Marinas de Andalucia

Puerto Real, Cádiz, España.


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