RE: [Histonet] Tissuearrays
Have you looked on my website. I have several articles in the journal
of histotechnology and some instructional videos on the same subjects
that go into detail on the subject of array construction.
My website is www.arrayworkshop.com
1. What I do is place the array block on a slide face down. Be sure
to put the slide on it before you flip the block over. Just in case
some of the cores fall out of the block depending on how loose the
2. Put the block ans slide (slide down) in an oven at approx. 37-40 'C
for 15 to 20 minutes.
3. Press the slide and block together. You will notice that the
paraffin will melt a little and the paraffin will spread out
slightly. This is good, because this is how the punches set into the
4. DO NOT seperate the slide and block at this time. Place then
together on an ice tray and allow to cool. The slide will seperate
easily once they are cold.
5. Before cutting, trim the sides a little to make them straight and
flat. This will help the ribboning to go smoother with flat edges.
6. Do not soak the block in ice water. I have found that ice water
makes the punches swell thus making the tissue mushey. Use only Ice.
If you feel you might get a better cut with water only soak for a
minute or so.
7. Use a fresh blade to make a ribbon. I even knock down the blade
with a Kim wipe to help the cutting go quickly. Running the kim wipe
across a new blade removes oils also. You will find the knife make
ribbons right away.
The rest is normal histology techniques. Lay the ribbons on the water
If you have any more questions please feel free to email me.
HT (ASCP) / Array Technician
>From: "Achstetter, Virginia A."
>Subject: [Histonet] (no subject)
>Date: Tue, 5 Oct 2004 13:45:11 -0400
>Does anyone have a good protocol for cutting Microarray blocks? I
understand that there are different methods of sealing the block cores
so they stay put when sectioning.
>Ginny Achstetter HT (ASCP)
>Armed Forces Institute of Pathology
>Soft Tissue Pathology
>6825 16th St. NW
>Bldg 54 Rm. 3062
>Washington, DC 20306
>Histonet mailing list
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