I am dealing with russel-movat-stain and with verhoeff-hematoxylin and have a question about the recipe.
In all books I have looked through, there is no HCl added except in John Kiernans Histological Methods.
In the Weigert's Hematoxylin we use also acidified ferric chlorid to stain nuclei. And I have learned that nuclei stain better in acid solution.
Now I have tried to stain elastic fibers with Verhoeff (AFIP-recipe) and after 30-45 min there was only a brown shadow.
Can anybody give me some hints, what was going wrong?
Is there a difference, if the Jodin-solution is made of Potassium-iodat instead of Potassium-iodide?
What about the acid in the solution?
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