[Histonet] quantum dots
This is a first-time post for me. Quantum dots (Qdots) are tiny fluorescent wafers similar to computer chips. The size of the dot determines the wavelength it emits and they are available in lots of different colors. While they excite at a broad range of wavelengths, you'll need different filters for optimum detection of the signal from the different colors. Look up the Quantum dot manual, available on their website, to see if you have the right filters for the two colors you'd like.
Qdots are far brighter than any fluorescent dye. I've used their Streptavidin-655, but had lower background with my own primary antibody conjugated to the 655 Qdots. The conjugation is quick and easy with their kit.
Alicia Henn, Ph.D.
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