[Histonet] orienting mouse embryos


Could I tap your collective experience to help solve a problem? I am trying
to orient gestational day 9.5 mouse embryos in paraffin for cross
sectioning. After processing, these specimens are like pieces of fragil
white pepper. I can only see head from tail with a microscope.  By the time
I get everything in focus the parffin is turning white....like finding a
ghost in a blizzard. Does anyone have any tips for this task?  Thanks, Lin

Department of Genetics, Cell Biology, and Anatomy
University of Nebraska Medical Center
Omaha, NE 68198-5455
Email: lkbauer@unmc.edu

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