[Histonet] micronucleus staining

From:"Fawn Jones"

Hi everyone,
Our histology labs is doing a wright-giemsa stain on mice micronucleus
slides and we are having a problem with our cells not picking up the
stain.  Are there any suggestions for us.
We let them dry overnight then we put them in Methanol for 5 minutes,
let them air dry for 20 minutes then they go into Wright's stain for 6
minutes, then Wright-giemsa stain for 6 minutes, and then go through 3
changes of sterile water for injection for 30 seconds each.  Then they
sit overnight before coverslipping.  If anyone could help that would be
Fawn Jones
North American Science Associates
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