[Histonet] fluorescent staining of nuclei or nuclear material

From:"Elizabeth Chlipala"

Hello everyone
I have a client that has some tissue (fascia) samples which have been
processed in an attempt to remove the intrinsic cells.  They have some
H&E sections, but want to use a more sensitive technique to ensure that
no cell remnants or nuclear material remains.  Is there is a fluorescent
staining method to visualize cell nuclear material.  I have gone to the
molecular probes web page and there is a wide variety of nuclear stains
available.  Does anyone have some advice on what would be the best
method for this application and whether or not it would work on paraffin
embedded material.
Thanks in advance.
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Histology Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
Ship to Address:
Premier Histology Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
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