We prepare our calcein for mouse injections as follows:  Dissolve 0.219 
grams NaCl in 25 ml distilled water.  Add 0.500 grams sodium bicarbonate 
and stir until dissolved.  Add 0.250g calcein powder slowly while gently 
heating above solution (be careful - it can foam up).  Stir until all 
calcein dissolves.  Filter sterilize.  Mouse dose of calcein is 30 mg/kg.

Nancy Troiano
Associate in Research III
Yale Core Center for Musculoskeletal Disorders
PO Box 208071
New Haven, CT 06520

(203)785-5136


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