[Histonet] Ventana Benchmark Attn: Vendors

From:Caldwell

Hi Everyone,
 
I am interested in obtaining quotes for Ventana's Benchmark for a client interested in doing HPV testing on Thin Prep paps.  Any information you could provide would be greatly appreciated.  Thank you in advance for your help.
 
Lourena
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Today's Topics:

1. Mailing list (Joseph Nerk)
2. Re: Mounting media to covberslip from ethano (Barbara Bublava)
3. elastic fibers (Jose Luis Palazon Fernandez)
4. Re: GI biosy embedding orientation (renton louise mrs)
5. IHC Equipment (Julie.Sanders@med.va.gov)
6. RE: GI biopsy embedding orientation
(Marshall Terry Dr, Consultant Histopathologist)
7. RE: IHC equipment (esteban enriquez)
8. Re: Dewaxing in IHC (esteban enriquez)
9. Calbindin (Inga Hansson)
10. RE: Mounting media to covberslip from ethano (Smith, Allen)
11. (no subject) (Bruijntjes, J.P.)
12. Re: Dewaxing in IHC (Gayle Callis)
13. Re: elastic fibers (Gayle Callis)
14. Tissue Storage Solutions (Etheridge, Sandra AGF:EX)
15. RE: GI biopsy embedding orientation (Bonner, Janet)
16. RE: (no subject) (Elizabeth Chlipala)


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Message: 1
Date: Sun, 03 Oct 2004 15:39:29 -0500
From: "Joseph Nerk" 
Subject: [Histonet] Mailing list
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain


My island was hit by a hurrican so that is why all messages sent to me
bounced as the internet system was down.
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1. http://g.msn.com/8HMBEN/2746??PS=47575


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Message: 2
Date: Mon, 4 Oct 2004 08:39:10 +0200
From: "Barbara Bublava" 
Subject: Re: [Histonet] Mounting media to covberslip from ethano
To: "Rena" , 
Message-ID: <000901c4a9dc$e1360690$1401a8c0@GERICHTS9XOZZ8>
Content-Type: text/plain; charset="iso-8859-1"

medite has a mounting medium called Mountex which can be used out of alcohol
or isopropanol. I am testing it right now and can not see drawbacks. but i
do not have expierience with longtime storage.

Barbara Bublava
----- Original Message ----- 
From: "Rena" 
To: 
Sent: Saturday, October 02, 2004 3:47 AM
Subject: [Histonet] Mounting media to covberslip from ethano


> Does anyone know of a mounting media that can be used to coverslip
> slides straight from ethanol and if so what are thw drawbacks.
>
> Rena Fail
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 3
Date: Mon, 4 Oct 2004 09:40:37 +0200 (CEST)
From: Jose Luis Palazon Fernandez 
Subject: [Histonet] elastic fibers
To: histonet@lists.utsouthwestern.edu
Message-ID: <20041004074037.0300F3127D5@perceval.uca.es>
Content-Type: text/plain; charset="iso-8859-1"

Dear List members

I would like to know, in your experience, what is the best method to stain elatic fibers. Any help would be appreciated.

thanks in advance

Josť Luis



------------------------------

Message: 4
Date: Mon, 04 Oct 2004 11:54:29 +0200
From: "renton louise mrs" 
Subject: Re: [Histonet] GI biosy embedding orientation
To: histonet@lists.utsouthwestern.edu
Message-ID: <1096883669.936be3a0rentonlf@bru.wits.ac.za>
Content-Type: text/plain; charset="UTF-8"

For many years the lab where i worked used to get GI biopsies on pieces of filter paper, carboard etc where they had been placed immediately after biosy and before immersion in formalin (they seemed to stick down of their own accord).We then processed them still stuck down. This was supposed to assist with orientation at embedding as they were initially placed mucosa up, and could thus be rotated as needed. I have always wondered if this is/done anywhere else.

louise




-----Original Message-----
From: "David Deibler" 
   
To: 
Date: Sat, 2 Oct 2004 07:18:14 -0500
Subject: [Histonet] GI biosy embedding orientation

Our lab processes many small gi bxs on a daily basis. Our pathologists have
been complaining about 

Incorrect orientation. We try to orient the specimens correctly but it
seems to be hit or miss.

Does anyone have suggestions or experience similar problems ???



David Deibler



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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
.......so what IS the speed of dark?



------------------------------

Message: 5
Date: Mon, 4 Oct 2004 06:16:10 -0500 
From: Julie.Sanders@med.va.gov
Subject: [Histonet] IHC Equipment
To: histonet@lists.utsouthwestern.edu
Message-ID:
<457381D92B01BD44B21CF37CC02EBDFD28E96F@vhacinexc2.v10.med.va.gov>
Content-Type: text/plain; charset="iso-8859-1"

Ventana Benchmark.

Julie Sanders,BA, HT(ASCP)
Supervisor, Anatomic Pathology
VAMC, Cincinnati, Oh.

--



------------------------------

Message: 6
Date: Mon, 4 Oct 2004 12:25:56 +0100
From: "Marshall Terry Dr, Consultant Histopathologist"

Subject: RE: [Histonet] GI biopsy embedding orientation
To: "David Deibler" 
   ,

Message-ID:

Content-Type: text/plain; charset="iso-8859-1"

The first point is that you must know *how* to embed them, which is at right angles to the curve or greatest curve if (as they usually are) they are curved in two planes. Aim for a section like this ...C.
The second point is that you need to be able to see this, and therein lies the rub. You certainly need an aid (magnification and a good light).

I have only seen this done in one place, Bristol Royal Infirmary, but they have the perfect solution. 
It is time consuming.

The apparatus is a test tube rack of plastic insulin syringes, with the nozzle cut off, so that they are a simple straight tube with a piston. These are put in the rack, piston down, and a space left above, in the barrel, for the tissue. Gelatine is put in and the specimen oriented under a dissecting microscope. When cool, the piston is pushed to remove the small pellet which is embedded in paraffin in the usual way.

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(Best I can do - these ascii artists must have infinite patience).


Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Consultant Pathologist
Rotherham General Hospital
South Yorkshire
England
terry.marshall@rothgen.nhs.uk

-----Original Message-----
From: David Deibler [mailto:ddeibler@grandecom.net]
Sent: 02 October 2004 13:18
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GI biosy embedding orientation


Our lab processes many small gi bxs on a daily basis. Our pathologists have
been complaining about 

Incorrect orientation. We try to orient the specimens correctly but it
seems to be hit or miss.

Does anyone have suggestions or experience similar problems ???



David Deibler



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 7
Date: Mon, 4 Oct 2004 04:46:38 -0700 (PDT)
From: esteban enriquez 
Subject: RE: [Histonet] IHC equipment
To: Joe Nocito , "GUTIERREZ, JUAN"
, "Baez, Janet"
, histonet@lists.utsouthwestern.edu
Message-ID: <20041004114638.61864.qmail@web61107.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Sorry to interrupt, but I heard that at NSH Vision
Biosystems had their Bondmax at their booth. Not an
impressive machine from the feedback as 1) you can't
do ISH, 2) there's no standard existing predilutes,
and 3) it's not a completely open system.

How does the Nemesis stack up??

Thanks Esteban
--- Joe Nocito wrote:

> Janet,
> I saw a new machine at the NSH from Biocare Medical
> called the Nemesis. It
> holds up to 84 slides, but the neat thing about it
> is that you can have
> programs running, and add more slides if you have to
> just like the Tekmate
> 1000. I miss my Tekmate because of the availability
> of adding more slides
> while the machine is running. Their numbe is
> 800-799-9499
> 
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu]On
> Behalf Of
> GUTIERREZ, JUAN
> Sent: Friday, October 01, 2004 3:23 PM
> To: Baez, Janet; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] IHC equipment
> 
> 
> Ventana's Benchmarks. The best in the business.
> 
> 
> Juan C. Gutierrez, HT(ASCP)
> Histology Laboratory Supervisor
> (210)704-2533
> 
> 
> -----Original Message-----
> From: Baez, Janet [mailto:jbaez@interscopepath.com]
> Sent: Tuesday, September 28, 2004 6:16 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] IHC equipment
> 
> It's been 5 years since we did IHC in-house. We use
> to run our IHC on
> the old TechMate 1000. We're thinking of bringing
> IHC back in-house.
> Any feed back as to the new equipment available.
> Any info will be
> greatly appreciated.
> Thanks.
> 
> Janet E. Baez
> Histology Manager
> Interscope Pathology Medical Group
> 21114 Vanowen St.
> Canoga Park, Ca.
> Tel. 818-992-7848
> Fax 818-992-6654
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 




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------------------------------

Message: 8
Date: Mon, 4 Oct 2004 04:50:10 -0700 (PDT)
From: esteban enriquez 
Subject: Re: [Histonet] Dewaxing in IHC
To: Phillip Huff , Gudrun Lang
, Histonetliste 
Message-ID: <20041004115010.35402.qmail@web61102.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Best thing I've heard used for dewaxing on certain
systems - which can also pull double duty as a cleaner
in tissue processors - is Isopar-L from Exxon-Mobil.
Dirt cheap by the 5 gallon tin or 55 gallon drum. This
is what Vision Biosystem use for dewax on their
Bondmax and as a cleaner on their Peloris.

Esteban


--- Phillip Huff wrote:

> We are currently using EZ-dewax from bioGenex. It
> works great, is re-usable until the solution is
> saturated with wax and is very good for the tissues.
> 
> http://www.biogenex.net/profile.php?pagename=autant1
> 
> Phil
> 
> Gudrun Lang wrote:
> Dear histonetters
> I would like to hear, what reagens is the most used
> in IHC for dewaxing the slides.
> We use xylen. And there is the opinion in our lab,
> that xylen is superior to other xylen-substitutes.
> Do you agree?
> For the HE-slides we use limonen, but for any
> delicate stain the boss demands xylen.
> thanks
> Gudrun
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
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------------------------------

Message: 9
Date: Mon, 4 Oct 2004 14:16:29 +0200
From: Inga Hansson 
Subject: [Histonet] Calbindin
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset="us-ascii" ; format="flowed"

Hi everyone

Has anyone used monoclonal anti-calbindin from Sigma on cryos? What 
fixation is best? PFA?

Thanks in advance!

Inga
-- 


Inga Hansson
dept. neuroscience, div. neurobiology
PO Box 587
Biomedical Centre
Husargatan 3
S-751 23
Uppsala
SWEDEN

phone:+46-18-4714384
fax: +46-18-559017



------------------------------

Message: 10
Date: Mon, 4 Oct 2004 08:54:19 -0400
From: "Smith, Allen" 
Subject: RE: [Histonet] Mounting media to covberslip from ethano
To: "Rena" 
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
<4C051EAE581BB646BF53A749A73FBA2D1F3BFB@exchsrv01.barrynet.barry.edu>
Content-Type: text/plain; charset="us-ascii"

Euparal, available from Carolina Biological Supply. The principal drawback
is that some stains will fade during long-term exposure to alcohol.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rena
Sent: Friday, October 01, 2004 9:47 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mounting media to covberslip from ethano



Does anyone know of a mounting media that can be used to coverslip
slides straight from ethanol and if so what are thw drawbacks.

Rena Fail
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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Barry University - Miami Shores, FL (http://www.barry.edu) 



------------------------------

Message: 11
Date: Mon, 4 Oct 2004 16:48:22 +0200
From: "Bruijntjes, J.P." 

Subject: [Histonet] (no subject)
To: 
Message-ID:
<3B070848E7C2204F9DEB8BCFD767728001079D26@ntexch1.voeding.tno.nl>
Content-Type: text/plain; charset="us-ascii"

Hi everyone



Is anyone of you aware of an antibody (mono- or polyclonal) that can be
used to detect apoptosis in rat colon FFPE?



J.P. Bruijntjes

TNO Nutrition and Food Research

Toxicology and Appllied Pharmacology

The Netherlands




This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html

------------------------------

Message: 12
Date: Mon, 04 Oct 2004 08:59:48 -0600
From: Gayle Callis 
Subject: Re: [Histonet] Dewaxing in IHC
To: "Gudrun Lang" , Histonet@lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20041004085428.01b250e8@gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

We have used both Clearite 3 (xylene substitute) from Richard Allen, and 
xylene. A key thing is to do enough changes and longer times in these 
solvents to insure good paraffin removal, plus we keep them relatively 
fresh (rotation is done more frequently to insure first solvent does not 
contain lots of paraffin aka contaminated after many sections going through 
prior to IHC slides.

We do 3 change, 5 minutes per change and either solvent for paraffin 
removal has worked well, no complaints from those doing IHC on paraffin 
sections.

For all other staining, we use Clearite 3, 2 changes at 3 minutes each, but 
once again, keep reagents rotated. Limonene makes too many people sick in 
our lab, not allowed on the premises.


At 08:26 AM 10/2/2004, you wrote:
>Dear histonetters
>I would like to hear, what reagens is the most used in IHC for dewaxing 
>the slides.
>We use xylen. And there is the opinion in our lab, that xylen is superior 
>to other xylen-substitutes. Do you agree?
>For the HE-slides we use limonen, but for any delicate stain the boss 
>demands xylen.
>thanks
>Gudrun
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)





------------------------------

Message: 13
Date: Mon, 04 Oct 2004 09:03:53 -0600
From: Gayle Callis 
Subject: Re: [Histonet] elastic fibers
To: Jose Luis Palazon Fernandez ,
Histonet@lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20041004090251.01b43550@gemini.msu.montana.edu>
Content-Type: text/plain; charset="iso-8859-1"; format=flowed

Verhoeff's van Giesons for coarser fibers, for finer fibers AND coarse 
fibers, Weigerts Resorcin Fuchsin.

At 01:40 AM 10/4/2004, you wrote:
>Dear List members
>
>I would like to know, in your experience, what is the best method to stain 
>elatic fibers. Any help would be appreciated.
>
>thanks in advance
>
>Josť Luis
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)





------------------------------

Message: 14
Date: Mon, 4 Oct 2004 08:09:15 -0700 
From: "Etheridge, Sandra AGF:EX" 
Subject: [Histonet] Tissue Storage Solutions
To: " (histonet@lists.utsouthwestern.edu)"

Message-ID: <424FAC08A8CDFE4BADD6497AE75B5E2F0E0A874B@atlas.gov.bc.ca>
Content-Type: text/plain

Hello, everyone,

I have a couple issues I was hoping to get some feedback on:

1. I work in the provicial agricultural animal health lab, and our
veterinary pathologists like to keep the original tissues from exotic
animals, whales, sea lions, other interesting cases, indefinitely. We are
currently transferring the tissues into 70% Ethanol, after about three
months in 10% NBF, for long term storage. Does anyone else keep tissue past
three months, and if so, what solution do you store them in? I have heard
that formalin will keep hardening the tissues and make IHC next to
impossible, and 70% will cause vacuolization of brain and may dehydrate over
time. I have used both at different facilities I have worked in.
Physiologic saline has been recommended, along with vacuum sealing the
tissues into bags. Any comments or recommendations?? I'm not really sure
why they want to hold on to the tissues, as we archive the blocks for future
use.

2. My lab director was at a conference down in Texas recently and someone

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