[Histonet] Staining rabbit mast cells
I am trying to identify mast cells in rabbit peripheral nerve. The tissue
is formalin fixed, postfiixed in osmium ( for myelin staining), and
embedded in Technivit 7100. I have tried a variety of acidic Toluidine
blue, alcian blue, safranin O, and thionin protocols, as well as an
alkaline thionin protocol from Bancroft, all of which are supposed to
identify mast cells. Areas of cartilage are staining as expected, but no
I am looking in the highly vascular connective tissue around the median
nerve. Some of the specimens have been compressed and show myelin
degeneration and extensive cellular reaction. Masts cells are probably
present. I plan to process rabbit skin as a control tissue, but have not
got the tissue yet.
Could the osmium be interfering with staining? I have tried both non osmium
stained gma and paraffin sections and still did not see mast cells. Do
rabbit mast cells stain differently from other species? I plan to try the
chloroacetate esterase kit from Sigma, but it might not work with osmium.
Any suggestions would be greatly appreciated.
University Califonia Medical Center
San Franscisco, Ca.
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